Update (06/24/2024): MFA Suite has new updates for: INCA, ETA and PIRAMID.   

The MFA Suite™ (Metabolic Flux Analysis Suite) is a software toolkit providing a powerful approach for quantifying the rates, or “fluxes”, of intracellular metabolic pathways.  The information generated from the MFA Suite of tools is useful for identifying pathway bottlenecks, elucidating network regulation, and quantifying the flow or fate of carbon within a biological system. By systematically accounting for all extracellular carbon inputs and outputs and all major intracellular pathways, MFA can be used to reconstruct comprehensive flux maps depicting cell metabolism.

The first MFA application, ETA, is provided as a set of MATLAB P-files via this website, and may be licensed and downloaded free of charge.

The second MFA application, INCA (Isotopomer Network Compartmental Analysis), is a MATLAB-based software package for isotopomer network modeling and metabolic flux analysis. Academic users may license and download the INCA application free of charge. Read more about INCA in the paper: INCA: a computational platform for isotopically non-stationary metabolic flux analysis, Jamey D. Young. Bioinformatics, first published online January 11, 2014.

MFA Suite has a new application, PIRAMID (Program for Integration and Rapid Analysis of Mass Isotopomer Distributions), which is a MATLAB-based software package that automates the quantification of metabolite mass isotopomer distributions (MIDs) from mass spectrometry datasets. Accurate MID data are critical inputs for metabolic flux analysis using INCA or other approaches that rely on stable isotopes to determine metabolic flux. Academic users may license and download the PIRAMID application free of charge.